Product Range


Product Range
 
Anil Bioplus Ltd. - A Pioneer in Bioplus in India
 
 

Manufacturing

Enzymes have been used for thousands of years to produce food and beverages, such as cheese, yoghurt, beer and wine. They were used in cheese manufacturing using yeasts and bacteria in food manufacturing. Enzymes which were isolated were first used in detergents earlier. The protein nature was discovered later and slowly the industrial applications also increased.

Enzyme production process can be divided into following phases:

  • Selection of an enzyme
  • Selection of a production strain
  • Construction of an overproducing strain by genetic engineering
  • Optimization of culture medium and production conditions
  • Optimization of recovery process (and purification if needed)
  • Formulation of a stable enzyme product

A Conventional fermentation process for enzyme production


Criteria used in the selection of an industrial enzyme include specificity, reaction rate, pH and temperature optima and stability, effect of inhibitors and affinity to substrates. Enzymes used in paper industry should not contain cellulose-degrading activity as a side activity because this activity would damage the cellulose fibres. Enzymes used in animal feed industry must be thermo tolerant to survive in the hot extrusion process used in animal feed manufacturing. The same enzymes must have maximal activity at the body temperature of the animal. Enzymes used in industrial applications must usually be tolerant against various heavy metals and have no need for cofactors. They should be maximally active already in the presence of low substrate concentration so that the desired reaction proceeds to completion in a realistic time frame.